Table 2.
Primers used in this study
| Genes and primers | Sequence (5′-3′) |
|---|---|
|
rimP homologues relevant primers |
|
| Lrimp-SC-F |
CCCAAGCTTGGCCAGCCGGTCCTCCAGTT |
| Lrimp-SC-R |
GCTCTAGAGGTGGTGCTCATCCGGGTGA |
| Rrimp-SC-F |
GGGGTACCAGGCCCACCACCCGCAGACT |
| Rrimp-SC-R |
GGAATTCCGGCGTGCGGCTTGGATCTA |
| CrimP-SC-F |
CGCGGCCAGTTCCTCACTGT |
| CrimP-SC-R |
CAGGGCGCTCATGTCGATGT |
| ECrimP-F |
TTGTCCACATTAGAGCAAAAATTAACAGAGATGATTACTGGAATTGTGAGCGGATAAC |
| ECrimP-R |
TCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTAGGCGATTAAGTTGGGTAA |
| YZECrimP-F |
TTGTCCACATTAGAGCAAA |
| YZECrimP-R |
TTAAAAGTGGGGAACCAG |
| PFrimP-F |
CGGGATCCGACCCACAACAGCACACG |
| PFrimP-R |
GCTCTAGATCTCCTTCTCCCGTACCAA |
| LrimP-SV-F |
AAGCTTGCCGAACGGTACAGAAAGGGTA |
| LrimP-SV-R |
TCTAGATCGCTCTGGGTGGTGCTCATC |
| RrimP-SV-F |
GGATCCGGCGAGTACGTCCTCGAAGT |
| RrimP-SV-R |
GATATCACCTTGCTCTCCACACCGAACTCC |
| CrimP-SV-F |
CGGCGGTTCGAAACCCATGC |
| CrimP-SV-R |
CTACGCCTCCTCTTCCTTCTTGTCCTT |
| YZrimP-SV-F |
GTGGTACGTCGTCGAAGATC |
| YZrimP-SV-R |
GCAAAGCGTCAGTCAACTTG |
| Primers for real-time PCR of genes in S. coelicolor |
|
| RTact-F |
GCTCCTCAGGCGGCACGA |
| RTact-R |
GCCGGCGGGTGTGGTACA |
| RTred-F |
GCCCTGACGCGCTATTGG |
| RTred-R |
GGTGGTGGGCGAGACGGA |
| RTcda-F |
GGAAAGCGACGCCTACTT |
| RTcda-R |
AGGCTCGTCTTTCCGATT |
| RTmetK-F |
CGAGCCCGTGGGTCTGTT |
| RTmetK-R |
CAGGTCGAGAGCGCGGAT |
| RTsigR-F |
CGACCACCTGCCCGACTC |
| RTsigR-R |
CCCCATGATGTCCGCGAT |
| RTsigE-F |
GGAGGAGGTGCCGACCGA |
| RTsigE-R |
TTCCCGCCGACATTCCGA |
| RTsigH-F |
GGAGCCGCTGGACGACCT |
| RTsigH-R |
CACCGCCCAGCCCTTGTC |
| RTbldN-F |
GACAGCGCCCGCATGATG |
| RTbldN-R |
GAGCGCCCGCAGAAAGGT |
| RTsigT-F |
GCCCTCGTCTCCGCCTAC |
| RTsigT-R |
CAGGCGTTCGGTGTCGTC |
| RTabsR1-F |
CCCGCAGTCGATCATGGA |
| RTabsR1-R |
GCAGGGCGAACTCCTTGTC |
| RTadpA-F |
AGCACCTCCACGAGCAGTTC |
| RTadpA-R |
CGTCCACCGAGTAGTCCGA |
| RTafsR-F |
GGCGGTGGATCTGCTGTG |
| RTafsR-R |
ACATCGCTGAGAACGGTGC |
| RTatrA-F |
CCGGCGGTGCGATGAGTA |
| RTatrA-R |
ACCCCAGCTCGCCGAACA |
| RTndgR-F |
CGACGTGACGGGCGAGAG |
| RTndgR-R |
GGAGCCGGCCTTCATGGT |
| RTphoP-F |
ACGTTCCCGTGATCATGGTG |
| RTphoP-R |
CAGTACGGCTCGGATGCG |
| RTrnc-F |
GGTGATCGGCGCGGTCTA |
| RTrnc-R |
CCTTCGGTCGCGGTGAGT |
| RTssgA-F |
CAGGCGCTGTTCCGTTCC |
| RTssgA-R |
GATGCGGTCCAGGGCCTC |
| RT5072-F |
GACGACCTGCCGCTCAAG |
| RT5072-R |
GAACGATGTGCGGTGGGT |
| RT5082-F |
GGAGGCCCTGGAGCAGTC |
| RT5082-R |
GCCGGCGATGATGATCTC |
| RT5086-F |
ACCTCACCGGCGTGTTCC |
| RT5086-R |
CGTGCTTCGAGGCGGAGT |
| RT5087-F |
GAACGACCGCCACGAGAC |
| RT5087-R |
GATCTCCAGCGAGCCGAT |
| RTsanG-F |
GGCCACCCTGCAGACGTAC |
| RTsanG-R |
CGGGACAGGTCGAACGTG |
| RTpolR-F |
GGTCTCCCGCGGACAACA |
| RTpolR-R |
GCGGCTCGTAGGACGTGA |
| RThrdB-SC-F |
GATCGCCGAGTCCGTCTC |
| RThrdB-SC-R |
CACTGAGTGGCCGGAATC |
| Primers for real-time PCR of genes in S. venezuelae |
|
| RTjadR3-F |
CACGTGGACGTGACGGATACGG |
| RTjadR3-R |
GGGTGTCGGCGAGGTTTCCTTC |
| RTjadW1-F |
TCGTCTGCTCCGACATCACCC |
| RTjadW1-R |
GCAGGAAGGAGACGCTCAGGTC |
| RTjadW2-F |
ACGTACTGATCCACTGCGCCTCC |
| RTjadW2-R |
CGATCAGGGAGTGCAGCGAGG |
| RTjadW3-F |
ACTACGGCAGCAACGAGAAGGC |
| RTjadW3-R |
AGGGCGAGGGTCATCGTGTC |
| RTjadR2-F |
TCGGCGATCAGTTCGGGAGC |
| RTjadR2-R |
AGCCATTCGCCGTTGTCCC |
| RTjadR1-F |
TGGACGGCTTGGAGGTCTGC |
| RTjadR1-R |
GGCTGCTCACATGGGTGTCG |
| RTjadJ-F |
CTGTCGGAGGCTCAGAACGC |
| RTjadJ-R |
ACGATCACGTTCGCAAGCAG |
| RTjadI-F |
TGCACAGCACTCTGATCGTGG |
| RTjadI-R |
GCGTTCGCCTCCCAGTTGTAG |
| RTjadA-F |
CCCCAACACCGTGGTCTCC |
| RTjadA-R |
GCGGTCGTTCTGCTTGGTG |
| RTjadB-F |
GGAGCCAGGGCAGCCAGTAC |
| RTjadB-R |
CGAAGGTGGAGCCGTATCCG |
| RTjadC-F |
GCAGCAAGACCTTCACCCTCG |
| RTjadC-R |
CCGACAGGTGCGCGTTGAC |
| RTjadE-F |
GCCGACGAGCTGTGGAACG |
| RTjadE-R |
GAGGCCAGGTAGCCGACGAG |
| RTjadD-F |
GCCTTGCTGCACGACTACCG |
| RTjadD-R |
ACGCCGTCCTCGTTCTCCTC |
| RTjadF-F |
ACGCCGCTCTGGGTGAACT |
| RTjadF-R |
GATGTCGAGTCCTGAGACCTTGC |
| RTjadG-F |
ACCTGACCGTCTTCAACCTCTTCG |
| RTjadG-R |
TGCTGCTGGTCCGGCTTCAC |
| RTjadH-F |
GACGACGACGCCGTGGAGA |
| RTjadH-R |
GATGTCCTCGCCCGTGATGC |
| RTjadK-F |
CGGCTGCGGACAGGAGTACG |
| RTjadK-R |
GAGGCCCAGGCTGATGTTGTG |
| RTjadL-F |
GGAAGGAGGAACGGAAGGACG |
| RTjadL-R |
ATCAGGGTGTAGAGGGCGAGG |
| RTjadM-F |
CCCGCTACACCGGAGTCCC |
| RTjadM-R |
GAGTCCCGTGCCGAGTCCC |
| RTjadN-F |
GCAGGGTTTCGGTCTGGAGG |
| RTjadN-R |
CGAGGCCGTTCTGGGTGATC |
| RTjadX-F |
CCACCACCGACCTCACCG |
| RTjadX-R |
CGAAGTGGGCGGAGGGC |
| RTjadO-F |
TTCCACAAGTCCAACCGCAAC |
| RTjadO-R |
TTCGATCAGCGGCTGGGTC |
| RTjadP-F |
AAGCACGTCCTGGCCGAGAAG |
| RTjadP-R |
GGTCCATGCCGAAGGCGATGT |
| RTjadQ-F |
CGACAAGCCGATGATCTACTACCC |
| RTjadQ-R |
GCGTGAGGTTCTTGGCGATGT |
| RTjadS-F |
GTCTTCCCGCCCAACCACG |
| RTjadS-R |
GCGAGGGAGCCAGCGTCAC |
| RTjadT-F |
CGACGAGGTGTACGGCACG |
| RTjadT-R |
CAGGATGCGTTCGGTCAGG |
| RTjadU-F |
CAGGTCAATCAGGTCAGCCACA |
| RTjadU-R |
CCGGTCGGACAGGATCAGC |
| RTjadV-F |
GACGAGCCGCAGGGCGAG |
| RTjadV-R |
CCGCTCCGCCACCATCCG |
| RThrdB-SV-F |
AGATTCCGCCAACCCAGTG |
| RThrdB-SV-R |
GAGCGTCGTCTCGTCTCGTC |
| other primers |
|
| rrnFp-F |
TGGAGGGAGATACGAGAACG |
| rrnFp-R |
CCCAGAGTGAAGGGCAGATT |
| MxylE-F |
TGAACCGAAGTGGATAAGTT |
| MxylE-R |
AGCCTTCAGATAGACACGGC |
| metK-F |
CGGCGGCTGGAATGAATGACCC |
| metK-R |
CAGGCCCGCGGCCTCGCGCA |
| sigR-F |
GGGCGGAGATCAGCCAGGAAAG |
| sigR-R |
TGACCCCGAGCCTTTCGCTTCGT |
| polB-F |
GGTGAAGACGCCAACGAC |
| polB-R |
GATCGGAGCGCGTACCAG |
| sanG-F |
GGGGTACCGTGCGTCAACCTCATCCCG |
| sanG-R |
GGAATTCGCTTGCCCGCTGGTCT |
| Kan-F |
TCTAGAGATCCCCTGGATACCGCTCG |
| Kan-R | GGATCCGTACCCGAACCCCAGAGTC |