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. 2013 Jul 3;6:93. doi: 10.1186/1754-6834-6-93

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Copyright © 2013 Reyes-Ortiz et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cellulose volume profiles are shown for chimeric cellulases with inactive catalytic domains (CD_KO-CBM). Amorphous cellulose films were incubated at room temperature with a) Cel9A_KO-CBM, and b) Cel5A_KO-CBM. Plots show the normalized cellulose volume fraction versus normalized distance from the silicon oxide surface. The profile for the film prior to enzyme addition is also shown. Arrows indicate the cellulose-buffer interface. Brown dotted line shows the extrapolation of the cellulose profile for Cel9A_KO-CBM after the enzyme layer at the surface of the film was removed.