Table 1.
Observations of cellulose films exposed to different cellulases
| Cellulase |
Percentage of mass/area remove |
Film swelling | |
|---|---|---|---|
| RT | Topt | ||
| Cel9A |
5 |
11 |
No |
| Cel9A-CBM |
12 |
32 |
Yes |
| Cel5A |
1 |
4 |
No |
| Cel5A-CBM | 16 | 35 | Yes |
Chimeric cellulases show higher cellulose removal and swelling of the amorphous cellulose film. The table above summarizes the neutron reflectivity (NR) results of the amorphous cellulose films exposed to several wild type and chimeric cellulases at room temperature. The mass/area removed is determined by integrating the cellulose volume profiles. Film swelling is the increase of film thickness above baseline observed after the film is exposed to the cellulase. RT and Topt stand for “room temperature” and “optimal temperature”, respectively. All standard deviations are within 10%.