Table 3.
Differentially expressed proteins in ROS- and ROS+ groups along with the spectral count and the spectral count ratio for the two groups
| Protein | NCBI # | SC ROS- | NSC-ROS- | SC ROS+ | NSC-ROS+ | NSC ratio in ROS+/ROS- |
|---|---|---|---|---|---|---|
| acetyl-Coenzyme A acetyltransferase 1 precursor (ACAT1) |
4557237 |
20 |
0.006 |
26.000 |
0.008 |
1.4 |
| acid phosphatase, prostate short isoform precursor (ACPP) |
6382064 |
17 |
0.005 |
27.000 |
0.009 |
1.7 |
| actin, alpha 1, skeletal muscle (ACTA1) |
4501881 |
0 |
0.000 |
8.000 |
0.003 |
ROS + only |
| A-kinase anchor protein 3 (AKAP3) |
21493041 |
76 |
0.022 |
70.000 |
0.022 |
1.0 |
| A-kinase anchor protein 4 isoform 2 (AKAP4) |
21493039 |
497 |
0.145 |
467.000 |
0.147 |
1.0 |
| angiotensin I converting enzyme 1 isoform 1 precursor (ACE) |
4503273 |
5 |
0.001 |
5.000 |
0.002 |
1.1 |
| ATP synthase, H + transporting, mitochondrial F1 complex, alpha subunit precursor (ATP5A1) |
4757810 |
28 |
0.008 |
30.000 |
0.009 |
1.2 |
| beta actin (ACTB) |
4501885 |
53 |
0.015 |
36.000 |
0.011 |
0.7 |
| brain creatine kinase (CKB) |
21536286 |
19 |
0.006 |
21.000 |
0.007 |
1.2 |
| chaperonin containing TCP1, subunit 4 (delta) (CCT4) |
38455427 |
4 |
0.001 |
6.000 |
0.002 |
1.6 |
| chaperonin containing TCP1, subunit 5 (epsilon) (CCT8) |
24307939 |
4 |
0.001 |
7.000 |
0.002 |
1.9 |
| chaperonin containing TCP1, subunit 8 (theta) |
48762932 |
5 |
0.001 |
4.000 |
0.001 |
0.9 |
| chromosome 20 open reading frame 3 (C20orf3) |
24308201 |
2 |
0.001 |
4.000 |
0.001 |
2.2 |
| clathrin heavy chain 1 (CLTC) |
4758012 |
14 |
0.004 |
6.000 |
0.002 |
0.5 |
| clusterin preproprotein (CLU) |
355594753 |
56 |
0.016 |
55.000 |
0.017 |
1.1 |
| CSE1 chromosome segregation 1-like protein (CSE1L) |
29029559 |
3 |
0.001 |
0.000 |
0.000 |
ROS- only |
| enolase 1 (ENO1) |
4503571 |
7 |
0.002 |
2.000 |
0.001 |
0.3 |
| eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) |
4503471 |
28 |
0.008 |
33.000 |
0.010 |
1.3 |
| eukaryotic translation elongation factor 2 (EEF2) |
4503483 |
12 |
0.003 |
5.000 |
0.002 |
0.5 |
| fatty acid synthase (FASN) |
41872631 |
40 |
0.012 |
35.000 |
0.011 |
1.0 |
| fibronectin 1 isoform 3 preproprotein (FN1) |
16933542 |
41 |
0.012 |
21.000 |
0.007 |
0.6 |
| fructose-bisphosphate aldolase A (ALDOA) |
4557305 |
15 |
0.004 |
17.000 |
0.005 |
1.2 |
| fumarate hydratase precursor (FH) |
19743875 |
5 |
0.001 |
7.000 |
0.002 |
1.5 |
| glutamine synthetase (GLUL) |
19923206 |
4 |
0.001 |
12.000 |
0.004 |
3.2 |
| glutathione peroxidase 4 isoform A precursor (GPX4) |
75709200 |
8 |
0.002 |
21.000 |
0.007 |
2.8 |
| glutathione S-transferase mu 3 (GSTM3) |
23065552 |
50 |
0.015 |
65.000 |
0.020 |
1.4 |
| glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
7669492 |
14 |
0.004 |
22.000 |
0.007 |
1.7 |
| glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS) |
7657116 |
210 |
0.061 |
185.000 |
0.058 |
1.0 |
| heat shock 70 kDa protein 2 (HSPA2) |
13676857 |
26 |
0.008 |
22.000 |
0.007 |
0.9 |
| heat shock 70 kDa protein 5 (HSPA5) |
16507237 |
5 |
0.001 |
11.000 |
0.003 |
2.4 |
| heat shock 90 kDa protein 1, alpha isoform 1 (HSP90AA1) |
153792590 |
39 |
0.011 |
26.000 |
0.008 |
0.7 |
| heat shock protein 90 kDa beta, member 1 (HSP90B1) |
4507677 |
11 |
0.003 |
22.000 |
0.007 |
2.2 |
| heat shock protein beta-1 (HSPB1) |
4504517 |
5 |
0.001 |
4.000 |
0.001 |
0.9 |
| histone cluster 1, H2aa (HIST1H2AA) |
25092737 |
47 |
0.014 |
56.000 |
0.018 |
1.3 |
| histone cluster 1, H2ae (HISTH1AE) |
10645195 |
14 |
0.004 |
13.000 |
0.004 |
1.0 |
| histone cluster 1, H2ba (HIST1H2BA) |
24586679 |
22 |
0.006 |
49.000 |
0.015 |
2.4 |
| lactotransferrin precursor (LTF) |
54607120 |
246 |
0.072 |
292.000 |
0.092 |
1.3 |
| lectin, mannose-binding 2 precursor |
5803023 |
3 |
0.001 |
5.000 |
0.002 |
1.8 |
| L-lactate dehydrogenase A isoform 1 (LDHA) |
5031857 |
10 |
0.003 |
9.000 |
0.003 |
1.0 |
| L-lactate dehydrogenase C (LDHC) |
4504973 |
17 |
0.005 |
12.000 |
0.004 |
0.8 |
| mitochondrial ATP synthase beta subunit precursor (ATP5B) |
32189394 |
50 |
0.015 |
70.000 |
0.022 |
1.5 |
| mitochondrial malate dehydrogenase precursor (MDH2) |
21735621 |
22 |
0.006 |
44.000 |
0.014 |
2.2 |
| olfactomedin 4 precursor (OLFM4) |
32313593 |
1 |
0.000 |
4.000 |
0.001 |
4.3 |
| outer dense fiber of sperm tails 1 (ODF1) |
194248060 |
15 |
0.004 |
14.000 |
0.004 |
1.0 |
| outer dense fiber of sperm tails 2 isoform 2 (ODF2) |
24430183 |
90 |
0.026 |
91.000 |
0.029 |
1.1 |
| peptidylprolyl isomerase A (PPIA) |
10863927 |
4 |
0.001 |
2.000 |
0.001 |
0.5 |
| peroxiredoxin 6 (PRDX6) |
4758638 |
4 |
0.001 |
1.000 |
0.0003 |
0.3 |
| phosphoglycerate dehydrogenase (PHGDH) |
23308577 |
3 |
0.001 |
5.000 |
0.002 |
1.8 |
| phosphoglycerate kinase 2 (PGK2) |
31543397 |
17 |
0.005 |
9.000 |
0.003 |
0.6 |
| phospholipase A2, group IIA precursor (PLA2G2A) |
4505849 |
6 |
0.002 |
10.000 |
0.003 |
1.8 |
| prolactin-induced protein (PIP) |
4505821 |
118 |
0.034 |
101.000 |
0.032 |
0.9 |
| prostate specific antigen isoform 1 preproprotein (KLK3) |
4502173 |
3 |
0.001 |
2.000 |
0.001 |
0.7 |
| protein disulfide-isomerase A3 precursor (PDIA3) |
21361657 |
7 |
0.002 |
7.000 |
0.002 |
1.1 |
| pyruvate kinase, muscle isoform M2 (PKM) |
33286418 |
49 |
0.014 |
31.000 |
0.010 |
0.7 |
| RAB2A, member RAS oncogene family (RAB2A) |
4506365 |
3 |
0.001 |
3.000 |
0.001 |
1.1 |
| ropporin (ROPN1) |
21359920 |
20 |
0.006 |
31.000 |
0.010 |
1.7 |
| ropporin, rhophilin associated protein 1B (ROPN1B) |
59891409 |
73 |
0.021 |
73.000 |
0.023 |
1.1 |
| saccharopine dehydrogenase (putative) (SCCPDH) |
55770836 |
13 |
0.004 |
16.000 |
0.005 |
1.3 |
| semenogelin I isoform a preproprotein (SEMG1) |
4506883 |
171 |
0.050 |
101.000 |
0.032 |
0.6 |
| semenogelin II precursor (SEMG2) |
4506885 |
513 |
0.149 |
226.000 |
0.071 |
0.5 |
| sorbitol dehydrogenase (SORD) |
156627571 |
36 |
0.010 |
27.000 |
0.009 |
0.8 |
| sperm acrosomal membrane protein 14 |
19424138 |
1 |
0.000 |
2.000 |
0.001 |
2.2 |
| sperm autoantigenic protein 17 (SPA17) |
8394343 |
15 |
0.004 |
20.000 |
0.006 |
1.4 |
| sperm protein associated with the nucleus, X chromosome, family member C (SPANX) |
13435137 |
54 |
0.016 |
80.000 |
0.025 |
1.6 |
| transglutaminase 4 (prostate) (TGM4) |
156627577 |
4 |
0.001 |
9.000 |
0.003 |
2.4 |
| triosephosphate isomerase 1 isoform 1 (TPI1) |
4507645 |
28 |
0.008 |
31.000 |
0.010 |
1.2 |
| tubulin alpha 6 (TUBA1C) |
14389309 |
9 |
0.003 |
11.000 |
0.003 |
1.3 |
| tubulin, alpha 3c (TUBA3C) |
17921993 |
117 |
0.034 |
134.000 |
0.042 |
1.2 |
| tubulin, beta 4 (TUBB4A) |
21361322 |
36 |
0.010 |
30.000 |
0.009 |
0.9 |
| tubulin, beta, 2 (TUBB4B) |
5174735 |
232 |
0.068 |
235.000 |
0.074 |
1.1 |
| tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide (YWHAZ) |
4507953 |
4 |
0.001 |
3.000 |
0.001 |
0.8 |
| valosin-containing protein (VCP) |
6005942 |
16 |
0.005 |
9.000 |
0.003 |
0.6 |
| voltage-dependent anion channel 2 (VDAC2) |
42476281 |
11 |
0.003 |
11.000 |
0.003 |
1.1 |
| voltage-dependent anion channel 3 isoform b | 25188179 | 7 | 0.002 | 12.000 | 0.004 | 1.9 |
A cut-off of 2-fold difference in NSC ratio was considered as significantly differentially expressed in ROS +. Overexpressed and underexpressed proteins are shown in bold.