Table 1.
Strains and plasmid used in this study
| Strains and plasmids | Relevant features | Reference |
|---|---|---|
|
E. coli strain | ||
| Novablue |
endA1 hsdR17(rK12-mK12+) supE44 thi-I gyrA96 relA1 lac recA1/F’[proAB+lacIq ZΔM15::Tn10(Tetr)] |
Novagen |
|
S. cerevisiae yeast strains | ||
| MT8-1 |
MATa ade leu2 his3 ura3 trp1 |
[18] |
| MT8-1/cocδBEC1 |
MATa ade leu2 his3 ura3, expressing β-glucosidase, endoglucanase and cellobiohydrolase genes on its cell surface |
[19] |
| MT8-1/δSWO |
MATa ade leu2 his3 trp1, expressing SWOI genes from T. reesei on its cell surface |
This study |
| MT8-1/δELPAO |
MATa ade leu2 his3 trp1, expressing AoelpI genes from A. oryzae on its cell surface |
This study |
| MT8-1/cocδBEC1/δSWO |
MATa ade leu2 his3, expressing β-glucosidase, endoglucanase, cellobiohydrolase, and SWOI genes on its cell surface |
This study |
| MT8-1/cocδBEC1/δELPAO |
MATa ade leu2 his3, expressing β-glucosidase, endoglucanase, cellobiohydrolase, and AoelpI genes on its cell surface |
This study |
| Plasmids | ||
| pδU-PGAGSWO |
URA3, expression of SWOI by δ-integration |
This study |
| pδU-PGAGELPAO | URA3, expression of AoelpI by δ-integration | This study |