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. 2013 Jul 13;11:62. doi: 10.1186/1477-7827-11-62

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Copyright © 2013 Hayashi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Protein localization of AM, CRLR, RAMP2 and RAMP3 in the bovine interplacentomal tissues on day 56 of gestation. Protein localization of (A and B) AM, (D and E) CRLR, (G and H) RAMP2 and (J and K) RAMP3 was detected by immunohistochemistry. (A, D, G and J) fetal membrane and endometrium. (B, E, H and K) blood vessels and uterine glands of the endometrium. Sections (7 μm) of the bovine interplacentomal tissues on Day 56 of gestation were incubated with anti-human AM, anti-human CRLR, anti-human RAMP2 and anti-human RAMP3 polyclonal antibodies. (C, F, I and L) no signal was detected in the negative control sections using normal rabbit IgG. The small panels within C, F, I and L show the portion of the blood vessels and uterine glands. FM, fetal membrane; LE, endometrial luminal epithelium; ST, stroma; BV, blood vessel; UG, uterine gland. Scale bars = 20 μm.