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. 2013 Jul 13;11:62. doi: 10.1186/1477-7827-11-62

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Copyright © 2013 Hayashi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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mRNA localization of AM, CRLR, RAMP2 and RAMP3 in the bovine placentome on day 230 of gestation. (A, D, G and J) Digoxigenin (DIG)-labeled anti-sense cRNA probes were used. (B, E, H and K) enlarged images of frames in (A, D, G and J) respectively. (C, F, I and L) DIG-labeled sense cRNA probes were used. Sections (7 μm) of bovine placentome on Day 230 of gestation were hybridized with each probe. AM mRNA (A and B) was detected in only BNCs. mRNA expression of CRLR (D and E), RAMP2 (G and H) and RAMP3 (J and K) was detected in cotyledonary villous and CEs. BNC, trophoblast binucleate cell; CE, caruncular epithelial cell; CV, cotyledonary villous. Scale bars = 20 μm.