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. 2013 Mar 13;3:16. doi: 10.1186/2191-219X-3-16

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Copyright ©2013 van den Hoff et al.; licensee Springer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Visualization of the difference between K_mand K_s. The plots cover a substantial range of the parameters k₂ and k₃, assuming a fixed ratio K₁ / k₂ = 0.7 ml/ml (a rationale for fixing this ratio is given in the discussion above). Top left, K_m; top right, V_r; bottom left, absolute difference (K_m - K_s); and bottom right, fractional difference ((K_m - K_s) / K_m). Parameters and their respective units: k₂, k₃ (1/min); V_r (ml/ml); and K_m, K_s (ml/min/100 ml). Moving along the line, V_r ≈ 0.55 ml/ml between K_m = 1 and 4 ml/min/100 ml corresponds approximately to the experimental data of Figure 2A.