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. 2013 Jul 11;14:68. doi: 10.1186/1471-2202-14-68

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Copyright © 2013 Ersland et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cellular localisation of the endogenous and recombinant LOC689986 protein. Human HeLa cells were transiently transfected with a vector encoding LOC689986 as a V5 tagged protein, or as a C- or N- terminally YFP tagged protein. After 24 hours the cells were subjected to V5- or YFP immunocytochemistry. The recombinant LOC689986 proteins are expressed in both the nucleus and the cytosol. The fluorescent micrographs depict nuclei (DAPI), expressed recombinant LOC689986 (LOC689986) and a merged image. Bars are indicated in the DAPI pictures.