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Flowchart of NURD algorithm. Using NURD to estimate the isoform expression mainly consists of five steps: (1) read mapping, which is not a part of NURD, but the preparation for it; (2) getting gene annotation; (3) counting reads based on gene annotation and read mapping; (4) getting likelihood function based on read counts and gene annotation; (5) estimating isoform expression by maximize the likelihood.
