Table 3.
Mean rate constants, equilibrium constants and coefficients of variation from the fit of Jones and Westbrook-type schemes
| Units | J&W, 3 binding sites (Scheme 1) | J&W, 4 binding sites | J&W, 5 binding sites (Scheme 2) | |
|---|---|---|---|---|
| α1 | s−1 | 2980 ± 20% | — | — |
| β1 | s−1 | 941 ± 65% | — | — |
| α2 | s−1 | 825 ± 20% | 2820 ± 16% | — |
| β2 | s−1 | 20,700 ± 47% | 934 ± 54% | — |
| α3 | s−1 | 6150 ± 34% | 832 ± 21% | 2780 ± 14% |
| β3 | s−1 | 180,000 ± 12% | 21,200 ± 44% | 922 ± 55% |
| α4 | s−1 | — | 5830 ± 30% | 849 ± 20% |
| β4 | s−1 | — | 180,000 ± 12% | 23,800 ± 43% |
| α5 | s−1 | — | — | 5530 ± 24% |
| β5 | s−1 | — | — | 180,500 ± 12% |
| d-1 | s−1 | 262,000 ± 99% | — | — |
| d+1 | s−1 | 96,900 ± 98% | — | — |
| d-2 | s−1 | 6010 ± 10% | 4060 ± 71% | — |
| d+2 | s−1 | 8030 ± 6% | 51,800 ± 97% | — |
| d-3 | s−1 | 18,400 ± 22% | 5840 ± 11% | 124,500 ± 98% |
| d+3 | s−1 | 1210 ± 38% | 7800 ± 6% | 6500 ± 72% |
| d-4 | s−1 | — | 18,700 ± 25% | 6370 ± 18% |
| d+4 | s−1 | — | 1230 ± 35% | 8290 ± 12% |
| d-5 | s−1 | — | — | 21,100 ± 32% |
| d+5 | s−1 | — | — | 1250 ± 28% |
| k-1 | s−1 | 1260 ± 30% | 342,000 ± 73% | 2460 ± 90% |
| k+1 | m−1 s−1 | (3.50 × 104) ± 23% | (1.57 × 106) ± 51% | (7.93 × 105) ± 47% |
| k-2 | s−1 | 2720 ± 31% | 1636 ± 45% | 34,125 ± 97% |
| k+2 | m−1 s−1 | (6.27 × 106) ± 45% | (1.77 × 107) ± 68% | (3.96 × 107) ± 98% |
| k-3 | s−1 | 1328 ± 27% | 1903 ± 32% | 442 ± 38% |
| k+3 | m−1 s−1 | (7.78 × 106) ± 48% | (8.33 × 106) ± 4% | (3.38 × 105) ± 28% |
| k-4 | s−1 | — | 1036 ± 26% | 1548 ± 35% |
| k+4 | m−1 s−1 | — | (2.33 × 107) ± 48% | (6.18 × 106) ± 46% |
| k-5 | s−1 | — | — | 875 ± 26% |
| k+5 | m−1 s−1 | — | — | (2.4 × 107) ± 47% |
| E1=β1/α1 | — | 0.37 ± 77% | — | — |
| E2=β2/α2 | — | 32 ± 58% | 0.37 ± 64% | — |
| E3=β3/α3 | — | 33 ± 19% | 33 ± 54% | 0.36 ± 65% |
| E4=β4/α4 | — | — | 34 ± 16% | 35 ± 51% |
| E5=β5/α5 | — | — | — | 34 ± 11% |
| D1=d+1/d−1 | — | 0.97 ± 40% | — | — |
| D2=d+2/d−2 | — | 1.35 ± 4% | 6.02 ± 79% | — |
| D3=d+3/d−3 | — | 0.09 ± 53% | 1 ± 5% | 0.73 ± 69% |
| D4=d+4/d−4 | — | — | 0.09 ± 52% | 1 ± 5% |
| D5=d+5/d−5 | — | — | — | 0.08 ± 49% |
| K1=k−1/k+1 | μm | 44,450 ± 40% | 461,760 ± 61% | 3240 ± 48% |
| K2=k−2/k+2 | μm | 820 ± 52% | 284 ± 78% | 1200 ± 15% |
| K3=k−3/k+3 | μm | 95 ± 46% | 223 ± 30% | 1200 ± 18% |
| K4=k−4/k+4 | μm | — | 71 ± 42% | 502 ± 55% |
| K5=k−5/k+5 | μm | — | — | 58 ± 40% |
| EC50 | μm | 305 ± 10% | 288 ± 6% | 284 ± 5% |
| nH | — | 2.5 ± 8% | 3.4 ± 5% | 3.7 ± 3% |
All the rate constants were fitted freely in HJCFIT. The values for efficacy at each level of ligation, E, the dissociation equilibrium constants per site, K, the equilibrium constants for the entry into the extra shut states, Dn, the EC50 and the Hill slope were calculated from the rate constants for each set and their means are listed at the bottom of the table. Values are means ± coefficient of variation (i.e. SD of the mean expressed as a percentage of the mean).