Table 4.
Mean rate constants, equilibrium constants and coefficients of variations from the fit of flip mechanisms with different numbers of binding sites
| Units | Flip, 3 binding sites (Scheme 3) | Flip, 4 binding sites | Flip, 5 binding sites (Scheme 4) | |
|---|---|---|---|---|
| α1 | s−1 | 2840 ± 30% | — | — |
| β1 | s−1 | 1230 ± 4% | — | — |
| α2 | s−1 | 498 ± 16% | 2690 ± 27% | — |
| β2 | s−1 | 18,800 ± 14% | 1220 ± 7% | — |
| α3 | s−1 | 2370 ± 28% | 496 ± 14% | 2520 ± 23% |
| β3 | s−1 | 154,000 ± 15% | 19,600 ± 13% | 1260 ± 7% |
| α4 | s−1 | — | 2030 ± 24% | 497 ± 13% |
| β4 | s−1 | — | 149,000 ± 16% | 20,700 ± 10% |
| α5 | s−1 | — | — | 1870 ± 22% |
| β5 | s−1 | — | — | 147,000 ± 16% |
| γ1 | s−1 | 1830 ± 23% | — | — |
| δ1 | s−1 | 91 ± 20% | — | — |
| γ2 | s−1 | 6180 ± 1% | 1950 ± 13% | — |
| δ2 | s−1 | 4280 ± 17% | 223 ± 23% | — |
| γ3 | s−1 | 2120 ± 27% | 5900 ± 5% | 2220 ± 13% |
| δ3 | s−1 | 17,500 ± 10% | 5060 ± 18% | 419 ± 31% |
| γ4 | s−1 | — | 2490 ± 26% | 5800 ± 7% |
| δ4 | s−1 | — | 14,500 ± 8% | 5820 ± 22% |
| γ5 | s−1 | — | — | 2550 ± 27% |
| δ5 | s−1 | — | — | 12,700 ± 5% |
| k- | s−1 | 241 ± 1% | 236 ± 15% | 222 ± 23% |
| k+ | m−1 s−1 | (7.32 × 104) ± 9% | (1.60 × 105) ± 6% | (2.45 × 105) ± 14% |
| kf- | s−1 | 4480 ± 9% | 5270 ± 13% | 5920 ± 16% |
| kf+ | m−1 s−1 | (9.88 × 106) ± 29% | (9.73 × 106) ± 24% | (9.73 × 106) ± 21% |
| E1=β1/α1 | — | 0.52 ± 30% | — | — |
| E2=β2/α2 | — | 41 ± 24% | 1 ± 28% | — |
| E3=β3/α3 | — | 70 ± 14% | 42 ± 22% | 1 ± 24% |
| E4=β4/α4 | — | — | 77 ± 11% | 44 ± 19% |
| E5=β5/α5 | — | — | — | 80 ± 8% |
| F1=δ1/γ1 | — | 0.05 ± 31% | — | — |
| F2=δ2/γ2 | — | 0.69 ± 16% | 0.11 ± 19% | — |
| F3=δ3/γ3 | — | 11 ± 45% | 1 ± 24% | 0.18 ± 20% |
| F4=δ4/γ4 | — | — | 7 ± 42% | 1 ± 29% |
| F5=δ5/γ5 | — | — | — | 6 ± 42% |
| KR=k−/k+ | μm | 3350 ± 11% | 1460 ± 10% | 885 ± 9% |
| KF=kf−/kf+ | μm | 263 ± 25% | 196 ± 19% | 159 ± 15% |
| EC50 | μm | 320 ± 8% | 300 ± 5% | 275 ± 7% |
| nH | — | 2.2 ± 5% | 2.7 ± 4% | 3.1 ± 3% |
The fits constrain the binding and unbinding rates to be the same, regardless of the number of molecules bound, in any conformation. The equilibrium constants, E, F and K, and open probability curve EC50 and the Hill slope, nH, were calculated from the rate constants for each set and then averaged. Values are means ± coefficient of variation (i.e. SD of the mean expressed as a percentage of the mean).