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. 2006 Feb 22;26(8):2227–2234. doi: 10.1523/JNEUROSCI.4329-05.2006

Figure 5.

Figure 5.

Umami-responsive cells express PLCβ2. A, Micrograph of a living slice preparation showing three cells filled with CaGD in two taste buds (dotted outlines). One of these cells (asterisk) responded to MPG plus IMP (next). The micrograph is pseudocolored to show CaGD fluorescence during live imaging (colors indicate pixel intensity mapping) (cf. Caicedo et al., 2000). A layer of CaGD fluorescent dye (dashed line) adheres to the surface epithelium and marks the mucosal boundary of the epithelium. B, The cell marked by the asterisk in A responded to focal stimulation of the apical tip of the taste bud with 200 mm MPG plus 1 mm IMP. None of the other CaGD-filled cells in the taste buds responded to MPG plus IMP. C–E, After immunostaining the preparation for PLCβ2, the same cell marked by the asterisk in A was relocated. C, The CaGD signal (green) is still visible in the three taste cells after immunostaining. D, PLCβ2 immunoreactivity (red) is present in many taste cells, including the recorded cell (asterisk). E, Signals from the PLCβ2 immunostaining and CaGD channels are merged. Many taste cells other than the recorded cell contain either PLCβ2 or CaGD, but not both. Scale bars, 10 μm.