(A) Naïve CD4+ T cells were cultured under modified Th1 cell-polarizing conditions as indicated (with anti-IL-4 included) for 3 days.
(B) Naïve CD4+ T cells from various strains were cultured under Th1 cell-polarizing conditions (IL-12 and anti-IL-4) in the presence of exogenous IFN-γ for 3 days.
(C-E) Various mouse strains were infected with T. gondii for 7 days. Cells harvested from spleens were characterized.
The expression of T-bet-ZsGreen was assessed by flow cytomteric analysis. IFN-γ production was assessed by intracellular staining after stimulation with PMA plus ionomycin (B) or plate-bound anti-CD3 plus anti-CD28 (E) for 4 hours. Histogram plots were gated on CD4+CD44hi cells. Numbers indicate % and the MFI of the positive cells in each gate. Error bars represent means+SD. Data are representative of three (A, B) and two (C-E) independent experiments.