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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Immunity. 2012 Oct 4;37(4):660–673. doi: 10.1016/j.immuni.2012.09.007

Figure 2. IL-12 and IFN-γ are redundant in inducing T-bet expression.

Figure 2

(A) Naïve CD4+ T cells were cultured under modified Th1 cell-polarizing conditions as indicated (with anti-IL-4 included) for 3 days.

(B) Naïve CD4+ T cells from various strains were cultured under Th1 cell-polarizing conditions (IL-12 and anti-IL-4) in the presence of exogenous IFN-γ for 3 days.

(C-E) Various mouse strains were infected with T. gondii for 7 days. Cells harvested from spleens were characterized.

The expression of T-bet-ZsGreen was assessed by flow cytomteric analysis. IFN-γ production was assessed by intracellular staining after stimulation with PMA plus ionomycin (B) or plate-bound anti-CD3 plus anti-CD28 (E) for 4 hours. Histogram plots were gated on CD4+CD44hi cells. Numbers indicate % and the MFI of the positive cells in each gate. Error bars represent means+SD. Data are representative of three (A, B) and two (C-E) independent experiments.