Figure 5. Suppression of Th2 cell-specific gene expression by T-bet is CD4⁺ T cell intrinsic.

(A) Naive CD4⁺ T cells from WT and Tbx21^−/− TBGR mice were sorted and transferred into Tcra^−/− mice. Three weeks later, splenocytes were harvested and cytokine production was assessed by intracellular staining after PMA plus ionomycin stimulation. % of cytokine producing cells was calculated within CD4⁺ZsG^neg and CD4⁺ZsG⁺ subsets. Error bars represent means+SD.

(B) Naïve CD4⁺ T cells were cultured under Th1 cell-polarizing conditions (IL-12 and anti-IL-4) for 3 days. IFN-γ and IL-4 production was assessed by intracellular staining after stimulation with PMA plus ionomycin. Numbers indicate % of cells in each quadrant.

Data are representative of two (A) and three (B) independent experiments.