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. 2013 Mar 15;9:479–485.

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Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.

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Figure 2. — Visualization of Cy5-LNA/DNA oligomer electrotransfer by direct confocal fluorescence microscopy. Plated Hela GFP-Rab7 cells were pulsed (0.3kV/cm, 10 pulses (p) of 5ms, 1Hz) on a glass coverslip chamber under a confocal microscope in presence of PI (100µM) (A) or Cy5-LNA/DNA oligomer (250nM) (B). Scan series were carried out before (0p), during (1p, 5p and 10p) and 10sec after (10p + 10sec) pulses application. The anode was located on the bottom of the pictures. Scale bare = 10µm. Pictures of PI and Cy5 fluorescence are in pseudo-color, GFP fluorescence is in green and gray pictures are light transmission acquisition. C. Intracellular localization of Cy5-LNA/DNA oligomers 2min after its application in non-pulsed cells (left picture) and in pulsed cells (right part). Scale bare = 10 µm. Cy5 and GFP fluorescence are in red and green respectively.