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. Author manuscript; available in PMC: 2013 Jul 21.
Published in final edited form as: Immunol Cell Biol. 2008 Nov 11;87(2):167–177. doi: 10.1038/icb.2008.80

Figure 6.

Figure 6

STAT-5 phosphorylation as a measurement of CD8+ T-cell responsiveness to IL-7. Purified CD8+ T cells were stained for IL-7Rα (CD127) expression or activated with the indicated concentrations of IL-7 for 30 min and stained for the phosphorylated form of STAT-5 (Y-649). (a) Representative histogram plots depict IL-7Rα expression on CD8+ T cells and P-STAT-5 staining relative to isotype control upon treatment with increasing concentrations of IL-7. (b) Quantitation of percentages of CD8+ T cells expressing P-STAT-5 and ΔMFI at each IL-7 concentration was tested. ΔMFI represents staining intensity with anti-P-STAT-5 minus isotype control antibody (Ab).