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. Author manuscript; available in PMC: 2013 Jul 21.
Published in final edited form as: Immunol Cell Biol. 2008 Nov 11;87(2):167–177. doi: 10.1038/icb.2008.80

Figure 7.

Figure 7

Within the CMV NLV-specific CD8+ T-cell pool, IL-7Rα expression correlates with STAT-5 phosphorylation upon IL-7 activation. Flow cytometry for CMV NLV-specific and IL-7Rα expression within CD8+ T cells. Purified CD8+ T cells were stained with (a) fluorescence isothiocyanate (FITQ-CD8 and phycoerythrin (PE)-HLA-A2/CMV pp65 tetramer or (b) FITC-CD127 and PE-HLA-A2/CMV pp65 tetramer. Overall, 2.5×l04 events were collected on the CD8/ lymphocyte gate to determine NLV-specific frequencies. Overall, 5×l03 events were collected on the tetramer/lymphocyte gate to evaluate CD127 expression. Representative dot plots are shown, and the percentages within the CD8 gate and tetramer gate are shown, (c) Cells were stained with HLA-A2/ CMV pp65 tetramers, incubated in media or IL-7 for 30min at 37°C and analyzed for STAT-5 phosphorylation. Overall, 5000 events were collected on the tetramer/lymphocyte gate. Representative dot plots are shown, and the percentages within the tet+ population are shown.