Table 1.

LCMSMS parameters, retention times (RT) and internal standards (IStd)

Analyte	MRM transitiona	DPb (V)	EPc (V)	CEPd (V)	CEe (eV)	CXPf (V)	RTg (min)
EME	200.2> 182.2	36	9	12	25	4	2.3
	200.2>82.2	36	9	12	35	4
EME-d3	203.3> 185.3	41	11	12	25	4	2.3
	203.3>85.2	41	11	12	35	4
OH-cotinine	193.2> 80.0	46	3.5	12	37	4	2.5
	193.2>134.0	46	3.5	12	27	4
OH-cotinine-d3	196.2> 80.0	46	10.5	14	43	4	2.5
	196.2>134.0	46	10.5	14	27	4
Cotinine	177.2> 80.1	41	10	12	33	4	4.1
	177.2>98.1	41	10	12	29	4
Cotinine-d3	180.2> 80.2	21	7	12	33	4	4.1
	180.2>101.2	21	7	12	31	4
Morphine	286.3> 152.0	66	3	16	71	4	5.8
	286.3>165.2	66	3	16	51	4
Morphine-d6	292.2> 201.0	61	3.5	22	25	4	5.8
	292.2>211.1	61	3.5	22	43	4
Codeine	300.3> 151.9	50	6.5	14	91	4	6.4
	300.3>165.1	50	6.5	14	53	8
Codeine-d6	306.3> 152.2	52	3.5	22	81	4	6.4
	306.3>165.2	52	3.5	22	53	4
6AM	328.2> 165.2	66	7.5	14	53	4	6.6
	328.2>152.2	66	7.5	14	79	4
6AM-d6	334.3 > 165.2	66	10	16	51	4	6.6
	334.3>152.0	66	10	16	80	4
BE	290.2> 168.3	41	9.5	22	25	4	6.9
	290.2>105.1	41	9.5	22	43	4
BE-d8	298.3> 171.3	46	3	20	27	4	6.9
	298.3>110.0	46	3	20	41	4
6AC	342.2> 225.5	71	4.5	20	41	4	7.3
	342.2>282.2	71	4.5	20	41	4
6AC-d3	345.2> 225.2	61	6	14	33	6	7.3
	345.2>285.2	61	6	14	27	4
Heroin	370.1> 268.2	76	7	14	39	4	7.3
	370.1 >328.1	76	7	14	35	4
Heroin-d9	379.2> 272.2	61	3.5	18	39	4	7.3
	379.2>335.3	61	3.5	18	37	4
NBUP	414.3> 340.1	86	12	26	43	4	7.5
	414.3>326.0	86	12	26	40	5
NBUP-d3	417.3 > 343.4	86	12	16	39	4	7.5
	417.3>326.0	86	12	22	43	4
Cocaine	304.3>182.3	51	3	16	25	4	7.6
	304.3> 82.1	51	3	16	37	4
Cocaine-d3	307.3> 185.3	41	5	18	27	4	7.6
	307.3>85.2	41	5	18	43	4
BUP	468.3> 396.2	86	12	18	53	6	8.4
	468.3>414.3	86	12	18	45	12
BUP-d4	472.5> 400.1	86	11	24	61	6	8.4
	472.5>415.3	86	11	24	45	8
EDDP	278.2>234.1	61	4	16	43	4	8.8
	278.2> 249.2	61	4	16	33	4
EDDP-d3	281.3> 234.1	61	9.5	24	31	4	8.8
	281.3>249.2	61	9.5	24	31	4
Methadone	310.3>265.2	31	4	14	19	4	8.9
	310.3> 223.3	31	4	14	27	4
Methadone-d3	313.3> 268.1	31	4.5	16	19	4	8.9
	313.3>226.2	31	4.5	16	27	4

MRM Multiple-reaction monitoring

^aThe quantification transition is rendered in italics

^bDeclustering potential

^cEntrance potential

^dCollision cell entrance potential

^eCollision energy

^fCollision cell exit potential

^gRetention time