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. Author manuscript; available in PMC: 2013 Jul 21.
Published in final edited form as: Dev Dyn. 2009 Aug;238(8):1964–1973. doi: 10.1002/dvdy.22030

Figure 3. Microprojection height of 5 μm does not attenuate mES cell-derived progeny proliferation.

Figure 3

EdU labeling of mES cells on flat (A) or T75-5 (B) PDMS membranes. P represents microprojection. Yellow circles are drawn 15 μm from microprojection edge and used to determine near/far parameter of cells. (C) Histogram showing influence microprojection height has on proliferation compared to cells grown on flat membranes. Green – EdU. Blue – 4,6-diamidino-2-phenylindole (DAPI). Green+blue gives turquoise color. Data are mean ± SE. * p< 0.01.