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SecA donor quenching and acceptor enhancement. Fluorescence spectra of AF568-labeled SecA-506C with unlabeled SecA-506C (red), AF568-labeled SecA-506C with AF647-labeled SecA-506C (black), and AF647-labeled SecA-506C with unlabeled SecA-506C (green). Observation of FRET is detected in the doubly-labeled sample as both a decrease in donor fluorescence and increase in acceptor fluorescence.
