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. Author manuscript; available in PMC: 2013 Jul 21.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Feb 12;393(4):643–648. doi: 10.1016/j.bbrc.2010.02.045

Figure 4.

Figure 4

A-D: Phospho-Akt and phospho-eNOS protein in HUVECs transfected with pre, or anti-miR-21. A: Western analysis (different parts of same blot) and densitometry of p-Akt (serine 473) after transfection with pre-miR-21 for 28 hours; *p=0.03 vs control transfection (n=3); B: Western (different parts of same blot) and densitometry of p-eNOS (serine 1177) after transfection with pre-miR-21 or control pre-miRNA for 48 hours, +/- LY 294002 (10 mM); *p=0.04 (n=6). C: Western analysis (different parts of same blot) and densitometry of p-Akt in cells transfected with anti-miR-21 or control, then subjected to USS (15 dynes/cm2) for 24 hours. D: p-eNOS expression (different parts of same blot) in cells transfected with anti-miR-21 or control, then subjected to USS (15 dynes/cm2) for 24 hours. *p=0.037 (n=3). E: NO production (expressed as ESR arbitrary units) in HUVECs transfected with pre-miR-21 or control, *p=0.007 (n=4).