Figure 9.

Chronic peroxide treatment of human neuronal cells reveals idiosyncratic AMI-derived plurigon structure and selective protein expression regulation. (A) Plurigon structures for AMI (panel 1), BDNF (panel 2) and NGF (panel 3)-mediated transcriptional activity in peroxide-treated SH-SY5Y cells. Panels 4–6 indicate the identical coordinate locations for the three plurigon structures in panels 1–3. The red COM vector line for each of the plurigon structures is depicted in panels 7–9. The COM vector line for the AMI plurigon is clearly distinct from the two more similar BDNF and NGF COM vector lines. (B) COM coordinate analysis for AMI, BDNF and NGF plurigon structures. AMI-derived plurigons demonstrate a significantly different r calculation (C), average radius from core (D) and surface area (E) compared to BDNF- and NGF-derived plurigon structures. AMI treatment (48 h) of peroxide-exposed SH-SY5Y cells engenders an idiosyncratic, and statistically distinct, cellular expression pattern, compared to BDNF and NGF, of MBOAT2 (F), NDUFB10 (G), NRAS (H), and TMED1 (I). Experiments in panels (F–I) were performed in triplicate and statistical significance was assessed using a Student's t-test (^*p < 0.05, ^**p < 0.01, ^***p < 0.001).