Methamphetamine and selenium alter GPx4 protein levels. Above: Representative western blot of GPx4 from SH-SY5Y cells grown in 0, 10, or 100 nM Se, with or without 100 μM Meth. Bars show mean ± SEM for four replicate cultures per condition. Below: Graph of GPx4 protein mean ± SEM measured by optical density of western blot bands. * indicates p<0.05, *** indicates p<0.001, and **** indicates p<0.0001 (Two-Way ANOVA with Bonferoni’s posthoc test).