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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Biomaterials. 2013 Jun 15;34(28):6938–6948. doi: 10.1016/j.biomaterials.2013.05.047

Figure 5. Transduction competence of substrate immobilized Ad vectors at delayed time points.

Figure 5

Figure 5

Figure 5

Figure 5

Ad-GFP was tethered to metal meshes via NHC, SHC, IHC or RHC and the meshes were placed on a confluent monolayer of BAEC. Two days post mesh placements, BAEC were trypsinized and removed without disturbing the meshes. New, non-transduced cultures of BAEC were then seeded onto the meshes. Ad-GFP transduction competency was measured by GFP expression through fluorescent microscopy (A) and fluorometry (B). Measurements were taken at indicated days, representative images were used and fluorometry results are means ± SEM, n = 4 Another set of Ad-GFP tethered meshes were incubated in complete cell growth medium at 37°C with mild shaking for 3 days prior to placements on a confluent monolayer of BAEC. Ad-GFP transduction competency was measured by GFP expression through fluorescent microscopy (C) and fluorometry (D). Measurements were taken at indicated days, representative images were used and fluorometry results are presented as means ± SEM, n = 4.