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. Author manuscript; available in PMC: 2013 Jul 22.
Published in final edited form as: J Med Chem. 2012 Jul 17;55(14):6554–6565. doi: 10.1021/jm300631e

Figure 4.

Figure 4

Direct activation of α1β3γ2L GABAA receptor with the enantiomers of barbiturate 14. Currents were elicited with increasing amounts of drug. With the exception of not using GABA, all conditions were the same as in Figure 3. Upper and lower traces show current responses to the stated concentrations of R-(−)-14 and S-(+)-14. For R-(−)-14 (circles): EC50 = 13 ± 8 μM, nH = 1.1 ± 0.4, Imax = 26 ± 5% (4 oocytes). For S-(+)-14 (squares): EC50 = 61 ± 6 μM, nH = 1.9 ± 0.2, Imax = 3 ± 0.1 % (one oocyte due to amount of material required). For least squares analysis, currents were normalized to the maximal GABA response for each oocyte.