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. 2012 Nov 6;3(11):1370–1385. doi: 10.18632/oncotarget.717

Figure 4. Targeting of ALK4 and MMP11 by miR-98.

Figure 4

(A) Potential binding sites of miR-98 was found in the 3'UTRs of ALK4 and MMP11. (B-C) Protein lysates were prepared from the 4T1 cells stably transfected with mir-98, anti-miR-98, or control GFP (B), and tumors formed by these cells (C). The lysates were subject to Western blot analysis for expression of ALK4 and MMP11. The same membranes were probed for actin expression to confirm equal loading. Expression of miR-98 repressed ALK4 and MMP11 levels while expression of anti-miR-98 played opposite effects. (D) The tumor sections were immunostained with anti-ALK4 and MMP11 antibodies. The miR-98 tumors exhibited lower levels of ALK4 and MMP11 than GFP tumors, while the anti-miR-98 tumors showed higher levels of ALK4 and MMP11 than GFP tumors. scale bars, 100 μm. (E-F) Human breast carcinoma specimens and the adjacent normal tissues were probed with anti-ALK4 and MMP11 antibodies. The tumor areas expressed higher levels of ALK4 and MMP11 than the normal tissues.