FIG. 3.
Leptin administration enhances the efficacy of islet transplantation for treatment of STZ-induced diabetes. Mice were treated with STZ on day −6 and subsequently received transplants of 50, 125, or 300 islets or sham surgery (day 0) and simultaneous osmotic pump implants delivering 1 µg/day leptin or vehicle for 6 weeks. A: The 4-h fasted blood glucose levels. B: Blood glucose data from days 5 to 43 analyzed by AUC. C: The 4-h fasted HbA1c levels in whole blood are presented as percents and mmol/mol equivalents are in parentheses. The 4-h fasted body weight gain normalized to day −1 (D) and net AUC from days 5 to 43 (E). F: The 4-h fasted plasma leptin measured from cardiac puncture samples collected 6 weeks after surgery. Data are presented as mean ± SEM (n = 3–5). *P < 0.05. †P < 0.05 vs. nondiabetic controls. ‡P < 0.05 vs. sham-vehicle controls. NS, not significant.