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. 2013 Jul 17;62(8):2843–2848. doi: 10.2337/db13-0160

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© 2013 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 1. — Heterochronic parabiosis model. A: One parabiosed partner was injected with BrdU and the joined pair was killed after 3 h. Both the injected mouse and attached mouse were stained for DNA (green) and BrdU (red). B: Parabiosed mice demonstrated a significant decline in body weight (*P < 0.05 for Student t test and ANOVA) in all three groups, after which their weight stabilized. C: Fed blood glucose levels of connected mice (all three groups) decreased significantly after 1 week (*P < 0.05 ANOVA) and remained lower throughout the experiment (N = 5). Het, heterochronic; Iso, isochronic.