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. 2013 Jul 17;62(8):2859–2869. doi: 10.2337/db12-1475

FIG. 3.

FIG. 3.

Blocking PD-1 results in sustained proliferation early during priming and causes quiescent cells to enter cycle late during the response. A: Representative flow cytometry histograms illustrating cell proliferation determined by CFSE dye dilution gated on Thy1.1+ BDC2.5 T cells from mice that received 250 μg/mouse isotype control or anti–PD-L1 antibody at days −3 and −1 relative to harvest at day 7 posttransfer. B: Frequency of BDC2.5 T cells that had fully diluted CFSE from A. Data are representative of three independent experiments with four mice in each treatment group. C: Ki67 expression from mice treated with 250 μg/mouse isotype control or anti–PD-L1 antibody at days −3 and −1 prior to harvest at day 42 posttransfer. D: Quantification of the frequency of Ki67+ BDC2.5 T cells from C. Data are representative of at least four independent experiments per organ with at least three mice per treatment condition. ingLN, inguinal LN; pancLN, pancreatic LN. ns, not significant, P > 0.05. *Significant, P = 0.01–0.05. **Very significant, P = 0.001–0.01.