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. 2012 Sep 28;3(10):1169–1181. doi: 10.18632/oncotarget.600

Figure 2. siRNA targeting JIP1 reduces JIP1 mRNA and protein expression in SaOS2 cells and causes increased sensitivity to doxorubicin.

Figure 2

(A) JIP1 mRNA silencing after transfection of JIP1 siRNA duplexes, analysed by RT-qPCR. JIP1 mRNA expression levels were normalised to GAPDH mRNA levels for each sample. Bars represent experiments performed in triplicate, error bars indicate SD. Results could not be obtained with JIP1 siRNA duplex #1 due to major cytotoxicity upon transfection of this siRNA. (B) Western blot analysis of JIP1 protein depletion at the indicated time-points after transfection of JIP1 siRNA duplex #2. (C) Dose-response curve of cells treated with doxorubicin (closed squares) and cells treated with doxorubicin after JIP1 gene silencing using JIP1 siRNA duplex #2 (open triangles). Results were obtained in an experiment performed in triplicate; error bars indicate SD. The JIP1 silenced cells show a significantly increased sensitivity to doxorubicin treatment (student's t-test at IC50; p < 0.0001).