Table 1. Primary screen hit list.

Accession number	Gene symbol	Gene name	p-value	t-statistic	FDR	Relative cytotoxicity
NM_000076	CDKN1C	cyclin-dependent kinase inhibitor 1C (p57, Kip2)	6.3E-05	−4.00	0.02	5.4
NM_005456	JIP1 (MAPK8IP1)	mitogen-activated protein kinase 8 interacting protein 1	1.0E-04	−3.89	0.02	8.6
NM_212469	CHKA	choline kinase alpha	1.1E-03	−3.26	0.12	5.7
NM_001319	CSNK1G2	casein kinase 1, gamma 2	3.7E-03	−2.90	0.22	2.7
NM_001570	IRAK2	interleukin-1 receptor-associated kinase-like 2	3.8E-03	−2.89	0.22	8.0
NM_001381	DOK1	docking protein 1, 62kDa (downstream of tyrosine kinase 1)	5.3E-03	−2.79	0.28	4.4
NM_001291	CLK2	CDC-like kinase 2	7.1E-03	−2.69	0.35	1.9
NM_020421	ADCK1	aarF domain containing kinase 1	9.1E-03	−2.61	0.41	1.3
NM_031284	ADP-GK	ADP-dependent glucokinase	0.011	−2.55	0.44	2.0
NM_004196	CDKL1	cyclin-dependent kinase-like 1 (CDC2-related kinase)	0.011	−2.54	0.44	3.6
NM_000586	IL2	interleukin 2	0.013	−2.48	0.49	5.2
NM_014826	CDC42BPA(a)	CDC42 binding protein kinase alpha (DMPK-like)	0.016	−2.42	0.49	11.1
NM_052947	HAK (ALPK2)	alpha-kinase 2	0.016	−2.42	0.49	1.1
NM_198452	PNCK	pregnancy up-regulated non-ubiquitously expressed CaM kinase	0.020	−2.33	0.54	3.0
NM_001025778	VRK3	vaccinia related kinase 3	0.021	−2.31	0.55	1.9
NM_001001329	PRKCSH	protein kinase C substrate 80K-H	0.024	−2.26	0.61	7.0

The top 16 genes that, upon silencing elicit a statistically significant (p < 0.025) increase in sensitivity to doxorubicin treatment in osteosarcoma cells with corresponding p-values and t-statistics for treatment effect, and false discovery rates for each gene. Relative cytotoxicity is defined as doxorubicin plus siRNA effect/doxorubicin effect.

a excluded from further analysis due to high direct cytotoxicity upon siRNA treatment alone