. Author manuscript; available in PMC: 2013 Jul 22.

Published in final edited form as: J Immunol. 2009 Aug 26;183(6):3661–3671. doi: 10.4049/jimmunol.0901218

Table I.

B lymphocyte frequencies and numbers

	Spleen		Peritoneal cavity
	B cells (x10⁻⁷)^a	MZ B cell frequency (%)^b	B cells (x10⁻⁵)^a	B cell frequency (%)^b
	B cells (x10⁻⁷)^a	MZ B cell frequency (%)^b	B cells (x10⁻⁵)^a	B-2	B-1a	B-1b

Wild type	5.1 ± 0.3	4.0 ± 0.1	7.0 ± 3.0	34 ± 7	26 ± 4	40 ± 3
CD21/35−/−	6.4 ± 0.9	5.2 ± 0.9	7.6 ± 0.8	32 ± 3	19 ± 3	49 ± 3
CD21/35^−/−CD19^+/−	6.3 ± 0.9	4.5 ± 1.0	5.2 ± 1.8	28 ± 6	17 ± 2	55 ± 4
CD 19−/−	1.7 ± 0.2^*	0.4 ± 0.1^*	2.3 ± 1.2	47 ± 5	6 ± 2^*	44 ± 4
CD21/35^−/−CD19^−/−	3.2 ± 0.6^*	0.9 ± 0.1^*	0.8 ± 0.2	44 ± 4	4 ± 1^*	52 ± 4

Mean B cell numbers (± SEM) were calculated based on the total number of B220⁺ cells harvested from spleen or the peritoneal cavity.

Values represent the mean frequency (± SEM) of lymphocytes expressing the indicated surface markers as determined by immunofluorescence staining with flow cytometry analysis.

The mean percentage or number of cells was significantly different from means of wild type mice, p<0.05. Results were from 4–6 mice of each genotype.