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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: J Med Chem. 2013 Jun 13;56(12):5059–5070. doi: 10.1021/jm400369q

Figure 2.

Figure 2

Direct inhibition of human α-thrombin by designed sulfated benzofuran trimers and a tetramer. The inhibition of thrombin was measured spectrophotometrically through hydrolysis of Spectrozyme TH in 20 mM Tris-HCl buffer, pH 7.4, containing 100 mM NaCl, 2.5 mM CaCl2 and 0.1 % PEG8000 at 25 °C. Solid lines represent fits to the data using equation 1 to obtain IC50, HS, YM, and YO, as described in ‘Experimental Procedures’.