Skip to main content
. Author manuscript; available in PMC: 2013 Jul 22.
Published in final edited form as: FEBS J. 2011 Aug 24;278(18):3319–3336. doi: 10.1111/j.1742-4658.2011.08248.x

Fig. 5. Recognition of rSra-HSP-17s by IgG in the serum from rSra-HSP-17s-immunized rats.

Fig. 5

A, B: The ELISA titration of the immunized sera against the pre-immune sera using rSra-HSP-17s proteins as the antigens. C, D: Western blot titration of the immunized sera using rSra-HSP-17s as the antigens tested with pre-immune serum (1:50) or rSra-HSP-17s-specific rat immune serum taken post immunization (in different dilutions). E, F: ELISA using the recombinant proteins as antigens and titration of the affinity-purified antibodies compared to the concentrated flow through.