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. Author manuscript; available in PMC: 2013 Jul 22.
Published in final edited form as: Pharm Res. 2010 May 26;27(8):1703–1712. doi: 10.1007/s11095-010-0170-2

Figure 3.

Figure 3

Excipients affect CYP3A4 expression in human primary hepatocytes. Human primary hepatocytes were prepared from two different donors (HL#14 and HL#17) and treated with RIF (10 μM), DMSO (0.1%), PBS (none) or selected excipients at concentrations indicated in Table 1 for 24 hrs mRNA (A) and 72 hrs protein (B) analysis, respectively. (A) Total RNA was isolated, reverse transcribed, then subjected to realtime RT-PCR analysis of CYP3A4 mRNA expression. (B) Whole cell homogenates (20 μg) from each group were subjected to immunoblot analysis of CYP3A4 protein expression as described in “Materials and Methods”.