FIGURE 4.

Effects of flow pattern on the activation of SREBP1 in a step flow chamber. (a) Shows side view of the step flow chamber in which confluent cultured BAECs are subjected to different flow patterns. After shearing for 1 or 12 h, the cells were fixed and immunostained for SREBP1. While disturbed flow induced a sustained activation of SREBP1, as indicated by its translocation into the nuclei (b, Lower left), laminar flow activated SREBP1 in a transient manner, as evidenced by the lack of nuclear staining of SREBP1 at 12 h (b, lower right). Modified from Liu et al. ²⁴