Fig. 1.

Elevated BR responses in bik1 mutant plants. (A) The bik1 mutant is partially insensitive to BRZ treatment. The seedlings of WT (Col-0), bak1-4, and bik1 mutants were grown in the dark for 8 d on 1/2 MS plates with or without 2 μM BRZ. (B) Quantification of hypocotyl length shown in A. (C) The bik1 mutant is hypersensitive to BL treatment. The seedlings were grown on 1/2 MS plates with or without 100 nM BL under the constant light for 14 d. (D) Quantification of root and hypocotyl length shown in C. The data are shown as mean ± SE from at least 25 seedlings. Asterisk indicates a significant difference with P < 0.05 compared with data from WT seedlings. (E) BIK1 complementation lines restore the BRZ insensitivity of the bik1 mutant. The above experiments were repeated three to four times with similar results.