Fig. 2.

oHSV can replicate in and kill 005 GSCs in vitro while expressing IL-12. (A) Infection and spread of G47Δ-mCherry (MOI = 0.1; red) in 005 GSC spheres (phase contrast). (B) Replication kinetics of G47∆-E and G47∆-mIL12 in 005 GSCs after infection at MOI = 0.1. (C) Dose–response curves for G47Δ-E and G47Δ-mIL12 in 005 GSCs after 4 d, as measured by MTS assay; average of two experiments. (D) Secreted IL-12 (culture supernatants) at different times after infection (MOI = 0.1, same experiment as B), as measured by ELISA. No detectable IL-12 was measured after G47Δ-E infection. Error bars indicate SEM. Experiments were performed in triplicate.