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. 2013 Jul 1;110(29):E2677–E2686. doi: 10.1073/pnas.1307235110

Fig. 1.

Fig. 1.

Actin polymerization activities of Sca2 constructs. (A) Domain organization of Sca2 and constructs used in this study (SS, signaling sequence; W, WH2 domains A, B and C; AC, autochaperone domain; TD, translocator domain. Constructs that were also made as Avi-tag fusions for Qdot labeling are highlighted in red. Constructs containing mutations in the proline-rich regions or WH2 domains are highlighted in magenta or maroon, respectively (the specific mutations are described in Fig. S2A). (B) Normalized time course of polymerization of 2 μM Mg-ATP-actin (6% pyrene-labeled) alone (black) or in the presence of 25 nM Sca2 constructs, as indicated. Initial polymerization rates were calculated between 0.1 and 0.3 of the normalized fluorescence and are shown in parenthesis. (C–F) Time course of polymerization of 2 μM Mg-ATP-actin (6% pyrene-labeled) alone (black) or with increasing concentrations of the Sca2 constructs shown in B (C), as well as constructs Sca421-670 (D), Sca34-1342 (E), and Sca1515-WABC (F), which are not shown in B.