Fig. 1.

Construction of an enhanced baculovirus vector for transduction of mammalian cells. (A) Baculovirus shuttle plasmids encoding a CMV-promoter driven gene expression cassette for mammalian cells were constructed from the pFastBac (Invitrogen) vector backbone. Additionally, pAcBac1 encodes a VSVG expression cassette for virus-pseudotyping and a WPRE element at the 3′-UTR of the transgene. (B) Expression of eGFP(wt) using pAcBac (Left) and pAcBac1 (Right) derived baculovirus in HEK293 cells. Cells were treated with the indicated virus at an MOI of 500, and the expression profile was analyzed 48 h postinfection. (C) FACS analysis of HEK293 cells infected with pAcBac1-GFPwt 48 h postinfection.