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. 2013 Jul 1;110(29):11976–11981. doi: 10.1073/pnas.1306180110

Fig. 2.

Fig. 2.

Dynein and NMII collaborate to polarize the centrosome in response to TCR photoactivation. Centrosome polarization in T cells treated with nontargeting (NT) shRNA or shRNA against DynHC (DHC), with or without 50 μM blebbistatin. (A) Representative time-lapse montages, with the time of UV irradiation indicated by yellow text. Yellow circles denote the irradiated region in each experiment. Time is indicated as minutes:seconds above the montages. (Scale bars: 5 μm.) (B) Average distance between the photoactivated region and the centrosome over time, with UV irradiation indicated by a purple line. (C) Maximum speed of centrosome movement (n ≥ 34 cells per sample). Error bars denote SEM. P values were calculated using Student t test. ***P < 0.001; **P < 0.01; *P ≤ 0.05; ns, P > 0.05.