Fig. 4.
Effect of reexpression of individual Mll targets on others in the network. RT-qPCR of genes in LSK cells reexpressing the cDNA indicated below each set of bars. Cells were produced in vivo by pI:pC injection, sorted 6 d later, then infected with a retrovirus without an added cDNA (empty) or cDNA as indicated. Two days later, retrovirally infected cells were sorted and RT-qPCR assays were performed. (A) Prdm16 expression levels in control MllF/F (blue) or Mll-deficient (red) LSK cells infected with the retrovirus indicated below each set of bars. Expression levels were normalized to the average expression level empty retrovirus-infected MllF/F cells and to Gapdh in each sample. Expression of Hoxa9 (B), Mecom transcripts (C), Pbx1 (D), and Eya1 (E) were analyzed and normalized as in A. Dashed lines indicate the average expression level in wild-type or Mll-deficient, empty retrovirus infected cells; four to five animals per genotype were used for each experiment, and error bars represent 95% CI. P values are shown for the comparison between pairs of empty vector and Evi1-expressing cells, calculated with the paired Student t test. ND, not detected.