Fig. 2.

Transgene imprinting is associated with differential allelic DNA methylation acquired in the germ line. (A) Methylation profiles on DNA from E12.5 presumptive digits of nonescaper HoxD^{Inv(rel5-Itga6)} Maternal/+ or +/Paternal embryos using transgene-specific primers (Table S1). (B) Same analysis using escaper embryos. The methylation rate is strongly reduced, with variations between samples reflecting the variable expression of the transgene. (C) Analysis of oocytes and sperm from the inverted allele shows germ line-acquired methylation. (D) Differential methylation over the transgene was not found in noninverted animals.