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Amide I response at a selected wavelength of 1,640 cm−1 (red, right scale) compared with that of a band at 1,491 cm−1 from the unlinked photoswitch (blue, left scale) and with that of the same band of the photoswitch linked to the PDZ2 domain (green, left scale). The signal of the photoswitchable PDZ2 domain (red and green) is fitted jointly to Eq. 1 (black).
