. Author manuscript; available in PMC: 2013 Jul 22.

Published in final edited form as: Biochem Biophys Res Commun. 2012 May 23;423(1):98–103. doi: 10.1016/j.bbrc.2012.05.089

Table 1.

Kinetic Parameters for AFC and a-Factor Photoaffinity Analogs as Substrates for His-Ste14p.

Compound	K_m(app)^a,^b	V_max^a,^b
	μM	pmol methyl groups transferred/min/mg
1	15.9 ± 0.9[17]	869.6 ± 14.9
2	17.9 ± 0.5	2072 ± 112
3	18.3 ± 0.4	362 ± 4.2
7a	14.6 ± 0.3	762 ± 28
7b	6.1 ± 0.6	320 ± 16
8a	15 ± 0.2	473 ± 8
8b	5.1 ± 0.6	240 ± 3.8

Data are the average of three experiments performed in duplicate ± standard deviation (S.D.)

Values were determined by fitting data to classical (Michaelis-Menten) enzyme-catalyzed curve data using GraphPad Prism 4.