Table 1.

Primers used for PCR amplification and/or cycle sequencing of plastid and nuclear DNA regions of the Platanus taxa sequenced in the study

Primer	Sequence (5′–3′)	Reference
Rps16 intron
Forward: rpsF	GTG GTA GAA AGC AAC GTG CGA CTT	Oxelman et al. (1997)
Reverse: rpsR2	TCG GGA TCG AAC ATC AAT TGC AAC	Oxelman et al. (1997)
TrnL(UAA) intron
Forward: c	CGA AAT CGG TAG ACG CTA CG	Taberlet et al. (1991)
Reverse: d	GGG GAT AGA GGG ACT TGA AC	Taberlet et al. (1991)
AtpB-rbcL intergenic spacer
Forward: oligo 2	GAA GTA GTA GGA TTG ATT CTC	Manen et al. (1994)
Reverse: oligo 5	TAC AGT TGT CCA TGT ACC AG	Manen et al. (1994)
TrnT(UGU)-trnL(UAA) intergenic spacer
Forward: PL_T1	GTA AAC TAC TGA GAT CTT AGC	This study
Reverse: PL_T2	AGA TAT TGA TGA TTG ATA ATC G	This study
PsbA-trnH(GUG) intergenic spacer
Forward: psbA3′f	GTT ATG CAT GAA CGT AAT GCT C	Sang et al. (1997)
Reverse: trnHf	CGC GCA TGG TGG ATT CAC AAT CC	Tate and Simpson (2003)
Forward : PL_psbA3′f_int ∼ 100	GTA AAA ACA TTA CTA CGG G	This study
Reverse: PL_trnHf_int ∼ 400	GGA ATT TCT ACC ATT CAT C	This study
LEAFY intron 2 (LFY-i2)
Forward: PlaLFY5	GTA ACT GGA CAT CCG ATC TGA TAT CC	Feng et al. (2005)
Reverse: PlaLFY4	AGC ATA GCA GTG CAC ATA GTG TCT C	Feng et al. (2005)
Forward: PL_L-ex2_F internal	AGG TTG GTA TGT GCT TGG C	This study
Internal transcribed spacer (ITS)
Forward: 18S (3′)	GGA GAA GTC GTA ACA AGG TTT CCG	Aceto et al. (1999)
Forward: 5·8S (3′)	TTG CAG AAT CCC GTG AAC CAT CG	Aceto et al. (1999)
Reverse: 26S (5′)	CCA AAC AAC CCG ACT CGT AGA CAG C	Aceto et al. (1999)
Reverse: 26S (5′) internal	TTC GCT CGC CGT TAC TAA GGG	This study