Table 2.

nrDNA SNP loci analysed with the qualitative assay and relative probes for LEAFY intron 2 (LFY-i2) and internal transcribed spacer 2 (ITS2)

Code	SNP	bp	Probe (5′–3′)	Length (bp)	Tm
LFY-i2*
LFY-1	G/A	421	T10-CGT TTG GCA GCC ACC TG	27	56 °C
LFY-2	T/G	550	T10-AGY TTT ACT TWA AAG CCT ACT AAA TA	36	56 °C
ITS2†
ITS-a	T/G	163	T5-CCC CCC GTG TGY CGT GT	22	58 °C
ITS-b	G/A	298	T16-GCC CCG GAG ACC CCG	31	58 °C

Further details about SNP sequence position are shown in *Supplementary Data File S2 and ^†Supplementary Data File S3.