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. 2012 Nov 28;32(48):17273–17286. doi: 10.1523/JNEUROSCI.3005-12.2012

Figure 6.

Figure 6.

Pharmacological block of CaV1.3a leads to less refined ribbon synapses in hair cells at 3 dpf. A, B, SR-SIM images of ribbon synapses in 3 dpf WT hair cells in larvae exposed to 0.1% DMSO (A) or 10 μm isradipine for 1 h (B). In isradipine-treated larvae, hair-cell synaptic ribbons appear enlarged and often misshapen. Scale bars, 1 μm. C, Average area of synaptic ribbons in control and isradipine (Israd.)-treated hair cells at 3 dpf. DMSO, 233 ± 12 nm2; isradipine, 293 ± 18 nm2; Mann–Whitney U test, *p = 0.0415. Error bars are SEM. D, Fraction of 3 dpf ribbon synapses within individual NMs with PSDs juxtaposing one, two, or three synaptic ribbons. Isradipine-treated NM hair-cell synapses contain two to three synaptic ribbons with slightly greater frequency than control (n = 4 NMs per condition, each containing ∼15–25 synapses). E, The shape factor of synaptic ribbons. Each spot represents an individual ribbon. Synaptic ribbons in hair cells are significantly less round in isradipine-treated larvae than control. Mann–Whitney U test, **p = 0.0051.