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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Exp Hematol. 2013 Feb 19;41(6):518–529.e5. doi: 10.1016/j.exphem.2013.02.006

Figure 4. Potential role of Hoxa9 co-factors in leukemic transformation.

Figure 4

Quantitative RT-PCR for A) Meis1 and B) Pbx3. Thymus from a healthy NHD13 mouse was used as a negative control for Meis1 and a positive control for Pbx3. All samples were calibrated to WT thymus set to 1. CH, clinically healthy. Mice 1077, 1118, 1125 and 1138 were mice with pre-T LBL. C) CFC re-plating assay with WT or Hoxa9 Lin- bone marrow transduced with empty vector (MIG) or Meis1. D) FACS analyses of re-plated transduced cells from (C) to assess for transduction efficiency and immunophenotype at 7th re-plating. Inset photomicrograph of Hoxa9 + Meis1 at 7th re-plating stained with May-Giemsa, scale bar = 100μm.